Bioss標(biāo)記抗體高分文獻(xiàn)引用精選
Ashok Kumar Jayavelu. et al. Splicing factor YBX1 mediates persistence of JAK2 -mutated neoplasms. Nature. 2020 Nov;588(7836):157-163.
《Nature》2020 Pubmed ID:33239784
產(chǎn)品編號:Anti-JAK2/Cy3 (bs-0908R-Cy3)
IF: 42.778 Application: IF Species: Mouse
Zhai, Yihui. et al. T lymphocyte membrane-decorated epigenetic nanoinducer of interferons for cancer immunotherapy. Nat Nanotechnol. 2021 Sep;:1-10.
《Nature Nanotechnology》2021 Pubmed ID:34580467
產(chǎn)品編號:Anti-LAMP2/FITC (bs-2379R-FITC)
IF: 39.213 Application: IF Species: Mouse
Alivernini S et al. Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis. Nat Med. 2020 Aug;26(8):1295-1306.
《Nature Medicine》2020 Pubmed ID:32601335
產(chǎn)品編號:Anti-MERTK/Cy3 (bs-0548R-Cy3)
IF: 36.13 Application: IF Species: Human
Emeric Limagne. et al. MEK inhibition overcomes chemoimmunotherapy resistance by inducing CXCL10 in cancer cells. Cancer Cell. 2022 Jan.
《Cancer Cell》2022 Pubmed ID: 35051357
產(chǎn)品編號:Anti-C-Myc/AF594 (bs-4963R-AF594)
IF: 31.743 Application: IF Species: Mouse
Dumont, Nicolas A., et al. "Dystrophin expression in muscle stem cells regulates their polarity and asymmetric division." Nature Medicine (2015).
《Nature Medicine》2015 Pubmed ID:26569381
產(chǎn)品編號:Anti-phospho-MARK2(Thr596)/AF647 (bs-5742R-AF647)
IF: 30.357 Application: IF Species: Mouse
Lu, Yiwen. et al. Eosinophil extracellular traps drive asthma progression through neuro-immune signals. Nat Cell Biol. 2021 Oct;:1-13.
《Nature Cell Biology》2021 Pubmed ID:34616019
產(chǎn)品編號:Anti-EAR1/AF555 (bs-1754R-AF555)
IF: 28.824 Application: IF Species: Human
Homan KA et al. Flow-enhanced vascularization and maturation of kidney organoids in vitro. Nat Methods. 2019 Mar;16(3):255-262.
《Nat Methods》2019 Pubmed ID:30742039
產(chǎn)品編號:Anti-VEGFR2/AF555 (bs-10412R-AF555)
IF: 28.467 Application: FCM Species: Human
Cai, Baohuan. et al. IRAK1-regulated IFN-γ signaling induces MDSC to facilitate immune evasion in FGFR1-driven hematological malignancies. Mol Cancer. 2021 Dec;20(1):1-20.
《Molecular Cancer》2021 Pubmed ID:34906138
產(chǎn)品編號:Anti-NKG2D/AF555 (bs-0938R-AF555)
IF: 27.401 Application: FCM Species: Mouse
Cheng Gao. et al. Surface-engineered chlorella alleviated hypoxic tumor microenvironment for enhanced chemotherapy and immunotherapy of first-line drugs. MATER TODAY. 2022 Jul.
《Materials Today》2022 Pubmed ID:10.1016/j.mattod.2022.06.024
產(chǎn)品編號:Anti-HIF-1 Alpha/AF555 (bs-0737R-AF555)
IF: 26.943 Application: IF Species: Mouse
Eszter á. Tóth. et al. Formation of a protein corona on the surface of extracellular vesicles in blood plasma. J Extracell Vesicles. 2021 Sep;10(11):e12140.
《Journal of Extracellular Vesicles》2021 Pubmed ID: 34520123
產(chǎn)品編號:Anti-Fibrinogen alpha chain/FITC (bs-7548R-FITC)
IF: 25.841 Application: Immune electron microscopy Species: Human
Li, Fengyin, et al. "Prostaglandin E1 and Its Analog Misoprostol Inhibit Human CML Stem Cell Self-Renewal via EP4 Receptor Activation and Repression of AP-1." Cell Stem Cell 21 (2017): 1-15.
《Cell Stem Cell》2017 Pubmed ID:28844837
產(chǎn)品編號:Anti-EP3/PTGER3/AF647 (bs-1876R-AF647)
Anti-Prostaglandin E Receptor EP1/AF647 (bs-6316R-AF647)
IF: 23.394 Application: FCM Species: Mouse
Yamamizu, Kohei, et al. "Protein Kinase A Determines Timing of Early Differentiation through Epigenetic Regulation with G9a." Cell stem cell 10.6 (2012): 759-770.
《Cell Stem Cell》2012 Pubmed ID: 22704517
產(chǎn)品編號:Anti-FOXA2/PE (bs-2358R-PE)
Anti-Nestin/PE (bs-0008R-PE)
IF: 22.634 Application: FCM Species: Mouse
Ni L et al. Detection of SARS-CoV-2-Specific Humoral and Cellular Immunity in COVID-19 Convalescent Individuals. Immunity 2020 06 16;526(6).
《Immunity》2020 Pubmed ID:32413330
產(chǎn)品編號:Goat Anti-Human IgM / HRP (bs-0345G-HRP)
IF: 22.553Application: ELISA Species: Human
Shogo Kumagaiet al. An Oncogenic Alteration Creates a Microenvironment that Promotes Tumor Progression by Conferring a Metabolic Advantage to Regulatory T Cells. Immunity. 2020 Jul 14;53(1):187-203.e8.
《Immunity》2020 Pubmed ID:32640259
產(chǎn)品編號:Anti-PPAR alpha/AF647 (bs-3614R-AF647)
Anti-PPAR Gamma/PE (bs-4590R-PE)
IF: 22.553 Application: FCM Species: Human
Lin Yao. et al. Nasal Spray of Neutralizing Monoclonal Antibody 35B5 Confers Potential Prophylaxis Against Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Variants of Concern (VOCs): A Small-scale Clinical Trial. CLIN INFECT DIS. 2022 Jun.
《CLINICAL INFECTIOUS DISEASES》2022 Pubmed ID:35666466
產(chǎn)品編號:Goat Anti-Human IgG H&L / HRP (bs-0297G-HRP)
IF: 20.999 Application: ELISA Species: Human
Miller A et al. ""Exploring Metabolic Configurations of Single Cells within Complex Tissue Microenvironments."" Cell Metabolism(2017)."
《Cell Metabolism》2017 Pubmed ID: 28889950
產(chǎn)品編號:Anti-FAPA/AF647 (bs-5758R-AF647)
Anti-CD31/AF488 (bs-0468R-AF488)
IF: 20.565 Application: IF Species: Human
van Ree JH et al. Pten regulates spindle pole movement through Dlg1-mediated recruitment of Eg5 to centrosomes. (2016) Nat.Cell.Bio. 18:814-21.
《Nat Cell Biol》2016 Pubmed ID:27240320
產(chǎn)品編號:Anti-SLC33A1/FITC (bs-0699R-FITC)
IF: 20.06 Application: IF Species: Mouse
標(biāo)記抗體服務(wù)
服務(wù)宗旨:高品質(zhì)的標(biāo)記原料采購,規(guī)范的標(biāo)記操作流程,嚴(yán)格的標(biāo)記產(chǎn)品質(zhì)控,合理的市場價格定位。
服務(wù)目標(biāo):以國際高標(biāo)準(zhǔn)的標(biāo)記服務(wù)水平,提供高品質(zhì)的標(biāo)記抗體產(chǎn)品,助力科研人員輕松科研。
一、服務(wù)內(nèi)容:
本服務(wù)項目主要為接收客戶的抗體、蛋白、多肽、小分子化合物進(jìn)行常規(guī)標(biāo)記物標(biāo)記。
1. 可接收的待標(biāo)記樣品包含抗體IgG、大分子蛋白、小分子多肽、小分子化合物等。
2. 可提供標(biāo)記服務(wù)的標(biāo)記物有:
A. 酶標(biāo)記物:堿性磷酸酶(AP/ALP),辣根過氧化物酶(HRP);
B. 生物素(Biotin);
C. 常規(guī)熒光素:FITC,Rhodamine B(RBITC);
D. Cy系列熒光素:Cy3,Cy5,Cy5.5, Cy7;
E. 熒光蛋白:藻紅蛋白(PE),別藻藍(lán)蛋白(APC),PerCP;
F. 熒光雙標(biāo)記:PE-Cy3,PE-Cy5,PE-Cy5.5,PE-Cy7;
G. 膠體金標(biāo)記Co-Au;
二、服務(wù)流程:
1. 客戶提交委托標(biāo)記請求并明確標(biāo)記要求,與公司商定具體的實驗項目、數(shù)量和費(fèi)用,雙方簽訂《委托標(biāo)記制備合同》。
2. 客戶提供待標(biāo)記樣品,我公司按照合同要求安排標(biāo)記服務(wù),并在規(guī)定時間內(nèi)完成標(biāo)記委托。
3. 標(biāo)記完成后,我公司按照客戶要求分裝,免費(fèi)低溫郵寄給客戶,同時提供標(biāo)記報告。
三、樣品要求:
1. 待標(biāo)記樣品需保證90%以上的純度,樣品中不含有干擾標(biāo)記物結(jié)合的成分,如有特殊成分,請?zhí)崆皹?biāo)注說明。
2. 待標(biāo)記樣品最小標(biāo)記量為1mg,最好是干粉,如果是液體,濃度應(yīng)大于1mg/ml。
3. 抗體IgG樣品中應(yīng)不含BSA、疊氮鈉、甘氨酸等成分。
4. 大分子蛋白應(yīng)提供分子量、等電點、緩沖液成分及pH等信息;小分子多肽需提供氨基酸序列;小分子化合物還需提供分子結(jié)構(gòu)式。
5. 請?zhí)崆罢f明任何特殊情況。
四、收費(fèi)標(biāo)準(zhǔn):
1. 抗體和大分子蛋白標(biāo)記收費(fèi)標(biāo)準(zhǔn):
標(biāo)記物
類型
標(biāo)記數(shù)量
周期
0.5-1mg
1-2mg
2-5mg
HRP
酶
1200
1600
2500
10天
AP
酶
2400
3600
6400
10天
Biotin
生物素
1200
1600
2500
10天
FITC
熒光
1200
1600
2500
10天
RBITC
熒光
1200
1600
2500
10天
Cy系列
熒光
1500
2200
3200
10天
AFluor
熒光
2400
3600
6400
10天
PE
熒光
2400
3600
6400
10天
APC
熒光
2400
3600
6400
10天
PerCP
熒光
3000
4800
-
10天
PE-Cy雙標(biāo)
熒光
3000
4800
-
15天
Co-Au
膠體金
1500
2500
4000
10天
五、標(biāo)記物介紹:
堿性磷酸酶(alkaline phosphatase,ALP或AP)是免疫診斷試劑產(chǎn)品最常用的標(biāo)記酶之一,優(yōu)點是穩(wěn)定性高、靈敏度高。ALP是一種能夠?qū)?yīng)底物去磷酸化的酶,即通過水解磷酸單酯將底物分子上的磷酸基團(tuán)除去,并生成磷酸根離子和自由的羥基,這類底物包括核酸、蛋白、生物堿等。而該脫去磷酸基團(tuán)的過程被稱為去磷酸化或脫磷酸化。堿性磷酸酶是磷酸酶的一種,磷酸酶的作用與激酶的作用正相反,激酶是磷酸化酶,可以利用能量分子,如ATP,將磷酸基團(tuán)加到對應(yīng)底物分子上。
辣根過氧化物酶(Horseradish Peroxidase,HRP)活性高,穩(wěn)定,分子量小,純酶容易制備,所以最常用。HRP廣泛分布于植物界,辣根中含量高,它是由無色的酶蛋白和棕色的鐵卟啉結(jié)合而成的糖蛋白,糖含量18%。HRP由多個同功酶組成,分子量為40,000,等電點為PH3~9,酶催化的最適PH因供氫體不同而稍有差異,但多在PH5左右。酶溶于水和58%以下飽和度硫酸銨溶液。HRP的輔基和酶蛋白最大吸收光譜分別為403nm和275nm,一般以O(shè)D403nm /OD275nm的比值RZ(德文ReinheitZahl)表示酶的純度。高純度的酶RZ值應(yīng)在3.0左右(最高可達(dá)3.4)。RZ值越小,非酶蛋白就越多。
FITC和RhodamineB(RBITC)是常見的熒光素,性質(zhì)較穩(wěn)定;
Cy系列熒光染料由于熒光較強(qiáng),對光猝滅有抵抗力而熒光不宜消退,近年來應(yīng)用比較廣泛;