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CD81 Rabbit pAb (bs-6934R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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產(chǎn)品編號(hào) bs-6934R
英文名稱 CD81 Rabbit pAb
中文名稱 CD81抗體
別    名 26 kDa cell surface protein TAPA 1; 26 kDa cell surface protein TAPA-1; 26 kDa cell surface protein TAPA1; CD 81; CD81 antigen; CD81 molecule; CD81_HUMAN; CVID6; S5.7; TAPA 1; Target of the antiproliferative antibody 1; Tetraspanin 28; Tetraspanin-28; Tetraspanin28; Tspan 28; Tspan-28; Tspan28.  
Specific References  (19)     |     bs-6934R has been referenced in 19 publications.
[IF=10.435] Li, Kanglu. et al. Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages. J Nanobiotechnol. 2022 Dec;20(1):1-20  WB ;  Human.  
[IF=9.518] Fan Y et al. High-sensitive and multiplex biosensing assay of NSCLC-derived exosomes via different recognition sites based on SPRi array. Biosens Bioelectron. 2020 Apr 15;154:112066.  WB&SPRi biosensor ;  Human.  
[IF=8.063] Faruqu FN et al. Membrane radiolabelling of exosomes for comparative biodistribution analysis in immunocompetent and immunodeficient mice–a novel and universal approach. Theranostics. 2019 Feb 28;9(6):1666-1682.  FCM ;  Mouse.  
[IF=7.711] Xiaoyi Gao. et al. Rolling Circle Amplification-Assisted Flow Cytometry Approach for Simultaneous Profiling of Exosomal Surface Proteins. Acs Sensors. 2021;6(10):3611–3620  FC ;  Human.  
[IF=7.31] Jingsong Chen. et al. Exploring the Temporal Correlation of Sarcopenia with Bone Mineral Density and the Effects of Osteoblast-Derived Exosomes on Myoblasts through an Oxidative Stress-Related Gene.. OXID MED CELL LONGEV. 2022 Sep;2022:9774570-9774570  WB ;  Mouse.  
[IF=6.639] Hyesun Jeong. et al. GCC2 as a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer. Cancers. 2021 Jan;13(21):5482  WB ;  Human.  
[IF=6.513] Jinglei Wang. et al. Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro. J CELL PHYSIOL. 2023 Apr;:  WB ;  Bovine.  
[IF=5.923] Samanta Mecocci. et al. Transcriptomic Characterization of Cow, Donkey and Goat Milk Extracellular Vesicles Reveals Their Anti-Inflammatory and Immunomodulatory Potential. Int J Mol Sci. 2021 Jan;22(23):12759  WB ;  Cow, Donkey,Goat.  
[IF=5.696] Jiang-Tao Fan. et al. Exosomal lncRNA NEAT1 from cancer-associated fibroblasts facilitates endometrial cancer progression via miR-26a/b-5p-mediated STAT3/YKL-40 signaling pathway. Neoplasia. 2021 Jul;23:692  WB ;  Human.  
[IF=5.195] Panpan Liu. et al. Endothelial progenitor cell-derived exosomes inhibit pulmonary artery smooth muscle cell in vitro proliferation and resistance to apoptosis by modulating the Mitofusin-2 and Ras-Raf-ERK1/2 signaling pathway. EUR J PHARMACOL. 2023 Apr;:175725  WB ;  Rat.  
[IF=4.889] Fengchun Liao. et al. ECFC-derived exosomal THBS1 mediates angiogenesis and osteogenesis in distraction osteogenesis via the PI3K/AKT/ERK pathway. J ORTHOP TRANSL. 2022 Nov;37:12  WB ;  Dog.  
[IF=4.85] Yutong Wu. et al. Osteoclast-derived extracellular miR-106a-5p promotes osteogenic differentiation and facilitates bone defect healing. CELL SIGNAL. 2022 Dec;:110549  WB ;  Mouse.  
[IF=4.757] Samanta Mecocci. et al. Antimicrobial and Immunomodulatory Potential of Cow Colostrum Extracellular Vesicles (ColosEVs) in an Intestinal In Vitro Model. BIOMEDICINES. 2022 Dec;10(12):3264  WB ;  Bovine.  
[IF=4.599] Shen, Xintong. et al. Evaluation of EpCAM-specific exosomal lncRNAs as potential diagnostic biomarkers for lung cancer using droplet digital PCR. 2021 Oct 14  WB ;  Human.  
[IF=4.546] Samanta Mecocci. et al. Anti-Inflammatory Potential of Cow, Donkey and Goat Milk Extracellular Vesicles as Revealed by Metabolomic Profile. Nutrients. 2020 Oct;12(10):2908  WB ;  Cow, Goat, Donkey.  
[IF=3.998] Jik-Han Jung. et al. Dual size-exclusion chromatography for efficient isolation of extracellular vesicles from bone marrow derived human plasma. Sci Rep-Uk. 2021; 11: 217  WB ;  Human.  
[IF=3.786] Yinfeng Chen. et al. PLA2G10 incorporated in exosomes could be diagnostic and prognostic biomarker for non-small cell lung cancer. Clin Chim Acta. 2022 Feb;:  WB ;  Human.  
[IF=3.281] Yinfeng Chen. et al. Serum exosomal hsa_circ_0069313 has a potential to diagnose more aggressive non-small cell lung cancer. Clin Biochem. 2022 Jan;:  WB ;  Human.  
[IF=0] Blans et al. Pellet-free isolation of human and bovine milk extracellular vesicles by size-exclusion chromatography. (2017) J.Extracell.Vesicle. 6:1294340  WB ;  Human.  
研究領(lǐng)域 細(xì)胞生物  免疫學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  細(xì)菌及病毒  淋巴細(xì)胞  b-淋巴細(xì)胞  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
克 隆 號(hào)
交叉反應(yīng) Human (predicted: Mouse,Rat)
產(chǎn)品應(yīng)用 WB=1:500-2000,Flow-Cyt=2ug/Test,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 26 kDa
檢測(cè)分子量 26
細(xì)胞定位 細(xì)胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human TAPA1/CD81: 101-210/236 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This protein appears to promote muscle cell fusion and support myotube maintenance. Also it may be involved in signal transduction. This gene is localized in the tumor-suppressor gene region and thus it is a candidate gene for malignancies. [provided by RefSeq, Jul 2008].

Function:
May play an important role in the regulation of lymphoma cell growth. Interacts with a 16-kDa Leu-13 protein to form a complex possibly involved in signal transduction. May acts a the viral receptor for HCV.

Subunit:
Plays a critical role in HCV attachment and/or cell entry by interacting with HCV E1/E2 glycoproteins heterodimer. Interacts directly with IGSF8. Interacts with CD53 and SCIMP.

Subcellular Location:
Membrane.

Tissue Specificity:
Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.

DISEASE:
Defects in CD81 are the cause of immunodeficiency common variable type 6 (CVID6); also called antibody deficiency due to CD81 defect. CVID6 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

Similarity:
Belongs to the tetraspanin (TM4SF) family.

SWISS:
P60033

Gene ID:
975

Database links:

Entrez Gene: 975 Human

Omim: 186845 Human

SwissProt: P60033 Human

Unigene: 54457 Human



產(chǎn)品圖片
Sample: Lane 1: Recombinant human CD81 protein, hFc (HEK293) Primary: Anti-CD81 (bs-6934R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kDa Observed band size: 36 kDa
Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-CD81 antibody (bs-6934R) Dilution: 2ug/Test; Secondary Antibody (white blue line) : Goat anti-rabbit IgG-AF488 Dilution: 0.5ug/Test. Isotype control(orange line):Normal Rabbit IgG Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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