| 產(chǎn)品編號(hào) | bs-0526R |
| 英文名稱 | Rabbit Anti-ALOX5 antibody |
| 中文名稱 | 5-脂氧合酶抗體 |
| 別 名 | 5-lipoxygenase; 5-LOX; 5 LO; 5LPG; ALOX 5; Arachidonate 5 lipoxygenase; Arachidonic acid 5 lipoxygenase; Leukotriene A4 synthase; LOG 5; LOG5; MGC163204; LOX5_HUMAN. |
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Specific References (5) | bs-0526R has been referenced in 5 publications.
[IF=5.895] Hui Wang. et al. Effect of Chlorogenic Acid via Upregulating Resolvin D1 Inhibiting the NF-κB Pathway on Chronic Restraint Stress-Induced Liver Inflammation. J AGR FOOD CHEM. 2022;70(34):10532–10542 WB, IHC ; Rat.
[IF=4.12] Wang et al. Kukoamine A inhibits human glioblastoma cell growth and migration through apoptosis induction and epithelial-mesenchymal transition attenuation. (2016) Sci.Rep. 6:36543 WB ; Human.
[IF=3.905] Xinmiao Wang. et al. Caffeic acid attenuates irradiation-induced hematopoietic stem cell apoptosis through inhibiting mitochondrial damage. Exp Cell Res. 2021 Dec;409:112934 FC ; Mouse.
[IF=1.72] Liang, Guojuan, et al. "The protective effect of caffeic acid on global cerebral ischemia-reperfusion injury in rats." Behavioral and Brain Functions 11.1 (2015): 1. WB ; Rat.
[IF=0] Wang, Ruiqi, et al. "The comparative study of acetyl-11-keto-beta-boswellic acid (AKBA) and aspirin in the prevention of intestinal adenomatous polyposis in APC Min/+ mice." Drug Discoveries & Therapeutics 8.1 (2014). IHC-P ; Mouse.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 轉(zhuǎn)錄調(diào)節(jié)因子 激酶和磷酸酶 合成與降解 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 78kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALOX5: 601-674/674 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants have been observed, but their full-length nature has not been determined. Function: Catalyzes the first step in leukotriene biosynthesis, and thereby plays a role in inflammatory processes. Subunit: Interacts with ALOX5AP and LTC4S. Subcellular Location: Cytoplasm. Nucleus matrix. Nucleus membrane; Peripheral membrane protein. Post-translational modifications: Serine phosphorylation by MAPKAPK2 is stimulated by arachidonic acid. Phosphorylation on Ser-523 by PKA has an inhibitory effect. Phosphorylation on Ser-272 prevents export from the nucleus. Similarity: Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain. SWISS: P48999 Gene ID: 240 Database links: Entrez Gene: 240 Human Entrez Gene: 11689 Mouse Omim: 152390 Human SwissProt: P09917 Human SwissProt: P48999 Mouse Unigene: 89499 Human Unigene: 41072 Mouse Unigene: 9662 Rat 合成與降解（Synthesis and Degradation） 5-脂氧合酶蛋白是花生四烯酸代謝途徑中的一種關(guān)鍵酶，在惡性腫瘤的發(fā)生、發(fā)展過(guò)程中起了重要作用。抑制5-脂氧合酶及其產(chǎn)物的表達(dá)有可能預(yù)防和逆轉(zhuǎn)惡性腫瘤的發(fā)生。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Lane 3: Rat Spleen tissue lysates
Primary: Anti-ALOX5 (bs-0526R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 78 kDa
Observed band size: 78 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ALOX5 5 lipoxygenase) Polyclonal Antibody, Unconjugated (bs-0526R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-5 lipoxygenase Polyclonal Antibody, Unconjugated(bs-0526R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control（black line）:A431.
Primary Antibody (green line): Rabbit Anti-ALOX5/5 lipoxygenase antibody (bs-0526R)
Dilution:1ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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