| 產(chǎn)品編號(hào) | bs-1074R |
| 英文名稱 | Rabbit Anti-Nrf2 antibody |
| 中文名稱 | 核因子2相關(guān)因子2抗體 |
| 別 名 | Nuclear factor-E2 related factor2; Nrf-2; HEBP1; NF E2 related factor 2; NFE2 related factor 2; NFE2L2; Nrf 2; Nuclear factor erythroid 2 related factor 2; Nuclear factor erythroid derived 2 like 2; NF2L2_HUMAN. |
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Specific References (123) | bs-1074R has been referenced in 123 publications.
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| 研究領(lǐng)域 | 腫瘤 免疫學(xué) 染色質(zhì)和核信號(hào) 合成與降解 |
| 抗體來(lái)源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Human,Mouse,Rat (predicted: Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | WB=1:100-1000,IHC-P=1:100-1000,IHC-F=1:100-1000,ICC/IF=1:100,IF=1:100-1000,ELISA=1:1000-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 68kDa |
| 細(xì)胞定位 | 細(xì)胞核 細(xì)胞漿 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Nrf2: 401-500/605 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項(xiàng) | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region. Subunit: Heterodimer. May bind DNA with an unknown protein. Interacts with KEAP1. Interacts via its leucine-zipper domain with the coiled-coil domain of PMF1. Subcellular Location: Cytoplasm, cytosol. Nucleus. Note=Cytosolic under unstressed conditions, translocates into the nucleus upon induction by electrophilic agents. Tissue Specificity: Widely expressed. Highest expression in adult muscle, kidney, lung, liver and in fetal muscle. Post-translational modifications: Phosphorylation of Ser-40 by PKC in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, promoting its translocation into the nucleus. Similarity: Belongs to the bZIP family. CNC subfamily. Contains 1 bZIP domain. SWISS: Q16236 Gene ID: 4780 Database links: Entrez Gene: 4780 Human Omim: 600492 Human SwissProt: Q16236 Human 轉(zhuǎn)錄調(diào)節(jié)因子（Transcriptin Regulators） 核細(xì)胞系因子2相關(guān)因子Nrf2，是一種對(duì)氧化還原反應(yīng)敏感的轉(zhuǎn)錄因子，可以通過正向促進(jìn)基因的表達(dá)，來(lái)轉(zhuǎn)錄翻譯產(chǎn)生一些抗氧化物，行使著異化解毒的酶類和藥物的高效外排泵作用。 有學(xué)者認(rèn)為：核因子2相關(guān)因子2蛋白在保護(hù)細(xì)胞免受環(huán)境毒性物質(zhì)影響方面發(fā)揮著極其重要的作用，它指導(dǎo)某些基因促進(jìn)細(xì)胞的防御工事，同時(shí)也刺激某些重要解毒酶對(duì)毒性物質(zhì)，如黃曲霉素的解毒和排毒作用。 |
| 產(chǎn)品圖片 |
Sample:
Lane 1: Kidney (Mouse) Lysate at 40 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: Liver ((Rat) Lysate at 40 ug
Lane 4: SiHa (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-Nrf2 (bs-1074R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 110’68 kD
Observed band size: 110 kD
Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Nrf2) Polyclonal Antibody, Unconjugated (bs-1074R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Tissue/cell: HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Nrf2)) polyclonal Antibody, Unconjugated (bs-1074R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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