| 產(chǎn)品編號 | bs-2430R |
| 英文名稱 | Rabbit Anti-Apelin Receptor antibody |
| 中文名稱 | 血管緊張素II受體樣蛋白1抗體 |
| 別 名 | APJ receptor; AGTR L1; AGTRL 1; AGTRL1; Angiotensin II receptor like 1; Angiotensin receptor like 1; Apelin receptor; APJ; B78; FLJ90771; G protein coupled receptor APJ; GPCR34; HG 11; HG11; MGC45246; MSR; msr/apj; Aplnr; Agtrl1; APLNR; FLJ96609; APJ_HUMAN. |
 |
Specific References (4) | bs-2430R has been referenced in 4 publications.
[IF=3.968] Bingyuan Ji et al. Roles for heterodimerization of APJ and B2R in promoting cell proliferation via ERK1/2-eNOS signaling pathway. Cell Signal. 2020 Sep;73:109671. co-IP ; Human.
[IF=2.976] Cemre Nur Balci. et al. The investigation of apelin and apelin receptor expressions in mouse endometrium during peri-implantation period. ANN ANAT. 2023 Feb;246:152027 WB, IHC ; Mouse.
[IF=1.64] Izgüt-Uysal, V. N., et al. "The effect of apelin on the functions of peritoneal macrophages." Physiological research (2017). IHC ; Rat.
[IF=1.61] Ustunel, Ismail, et al. "The effects of water immersion and restraint stress on the expressions of apelin, apelin receptor (APJR) and apoptosis rate in the rat heart." Acta Histochemica (2014) IHC-P ; Rat.
|
| 研究領域 | 心血管 細胞生物 免疫學 G蛋白偶聯(lián)受體 G蛋白信號 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應 | Human,Mouse (predicted: Rat,Rabbit,Dog) |
| 產(chǎn)品應用 | WB=1:500-2000,Flow-Cyt=1μg/Test,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 42kDa |
| 細胞定位 | 細胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Apelin receptor: 41-140/380 <Cytoplasmic> |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq Function: Receptor for apelin coupled to G proteins that inhibit adenylate cyclase activity. Alternative coreceptor with CD4 for HIV-1 infection; may be involved in the development of AIDS dementia. Subcellular Location: Cell membrane. Tissue Specificity: Widely expressed in the brain, in glial cells, astrocytes and neuronal subpopulations, as well as in the spleen, thymus, ovary, small intestine and colon. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: P35414 Gene ID: 187 Database links: Entrez Gene: 429337 Chicken Entrez Gene: 187 Human Entrez Gene: 23796 Mouse Omim: 600052 Human SwissProt: P35414 Human SwissProt: Q9WV08 Mouse Unigene: 438311 Human Unigene: 29368 Mouse Unigene: 7965 Rat |
| 產(chǎn)品圖片 |
Sample:
Stomach (Mouse) Lysate at 40 ug
Primary: Anti-Apelin Receptor (bs-2430R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: Heart (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Cerebrum (Rat) Lysate at 40 ug
Lane 5: Spinal cord (Mouse) Lysate at 40 ug
Lane 6: Spinal cord (Rat) Lysate at 40 ug
Primary: Anti-Apelin Receptor (bs-2430R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 50 kD
Sample:
Cerebrum(mouse) Lysate at 40 ug
heart(mouse) Lysate at 40 ug
Primary: Anti-Apelin receptor (bs-2430R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 52 kD
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-Apelin Receptor antibody (bs-2430R)
Dilution:1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Negative control（white blue line）: PBS
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Positive control(blue): mouse Splenocytes
Isotype Antibody (orenge): Rabbit IgG, Dilution: 1ug in 100 u1 X PBS containing 0.5% BSA
Secondary Antibody(light blue): Goat anti-rabbit IgG-FITC,Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody catalog number (green): bs-2430R, Dilution: 1ug in 100ul 1X PBS containing 0.5% BSA
|
| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
