| 產(chǎn)品編號 | bs-3808R |
| 英文名稱 | Rabbit Anti-Radixin antibody |
| 中文名稱 | 根蛋白抗體 |
| 別 名 | ESP10; Hh-induced MATH and BTB domain-containing protein; HIB; Moesin-B; Protein roadkill; Radixin; rdx; CB567; CG12537; DFNB24; RADI_HUMAN. |
| 研究領(lǐng)域 | 細(xì)胞生物 免疫學(xué) 細(xì)胞表面分子 上皮細(xì)胞 |
| 抗體來源 | Rabbit |
| 克隆類型 | Polyclonal |
| 交叉反應(yīng) | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 產(chǎn)品應(yīng)用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理論分子量 | 69kDa |
| 細(xì)胞定位 | 細(xì)胞漿 細(xì)胞膜 |
| 性 狀 | Liquid |
| 濃 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Radixin: 25-130/583 |
| 亞 型 | IgG |
| 純化方法 | affinity purified by Protein A |
| 緩 沖 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存條件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 產(chǎn)品介紹 |
Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. It is highly similar in sequence to both ezrin and moesin. The radixin gene has been localized by fluorescence in situ hybridization to 11q23. A truncated version representing a pseudogene (RDXP2) was assigned to Xp21.3. Another pseudogene that seemed to lack introns (RDXP1) was mapped to 11p by Southern and PCR analyses. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. Function: Radixin is a cytoskeletal protein that may be important in linking actin to the plasma membrane. Subunit: Binds SLC9A3R1. Interacts with NHERF1, NHERF2, LAYN, MME/NEP and ICAM2 Subcellular Location: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cleavage furrow. Note=Highly concentrated in the undercoat of the cell-to-cell adherens junction and the cleavage furrow in the interphase and mitotic phase, respectively. Post-translational modifications: Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-binding DISEASE: Defects in RDX are the cause of deafness autosomal recessive type 24 (DFNB24) [MIM:611022]. DFNB24 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Similarity: Contains 1 FERM domain. SWISS: P35241 Gene ID: 5962 Database links: Entrez Gene: 5962 Human Entrez Gene: 19684 Mouse Omim: 179410 Human SwissProt: P35241 Human SwissProt: P26043 Mouse Unigene: 263671 Human Unigene: 592679 Human Unigene: 245746 Mouse Unigene: 472057 Mouse Unigene: 224619 Rat 根蛋白是一種細(xì)胞骨架蛋白，在連接肌動蛋白于質(zhì)膜中可能有重要作用。研究表明，根蛋白對于結(jié)合膽紅素通過其Mrp2在BCMs中定位支持的分泌是需要的。埃茲蛋白-根蛋白-膜突蛋白家族蛋白是亞膜皮質(zhì)，特別是上皮細(xì)胞中普遍存在的成分。其可能參與將肌動蛋白絲的頂端錨定于胞膜。 |
| 產(chǎn)品圖片 |
Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Radixin) Polyclonal Antibody, Unconjugated (bs-3808R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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