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Rabbit Anti-CR2  antibody (bs-3792R)
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產(chǎn)品編號 bs-3792R
英文名稱 Rabbit Anti-CR2  antibody
中文名稱 2型補體受體(CD21)抗體
別    名 C3DR; CD 21; CD21; CD21 antigen; Complement C3d receptor; Complement component (3d/Epstein Barr virus) receptor 2; Complement component receptor 2; Complement receptor type 2; Cr 2; EBV receptor; Epstein Barr virus receptor; CR2_HUMAN.  
研究領域 細胞生物  免疫學  細胞膜受體  細菌及病毒  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應 Human,Rat (predicted: Mouse)
產(chǎn)品應用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg/Test,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 111kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human CR2: 981-1092/1092 <Cytoplasmic>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMed PubMed
產(chǎn)品介紹 This gene encodes a membrane protein, which functions as a receptor for Epstein-Barr virus (EBV) binding on B and T lymphocytes. Genetic variations in this gene are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9). Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Function:
Receptor for complement C3Dd, for the Epstein-Barr virus on human B-cells and T-cells and for HNRPU. Participates in B lymphocytes activation.

Subunit:
Interacts (via Sushi domain 1 and 2) with C3dg.

Subcellular Location:
Membrane; Single-pass type I membrane protein.

Tissue Specificity:
Mature B-lymphocytes, T-lymphocytes, pharyngeal epithelial cells, astrocytes and follicular dendritic cells of the spleen.

DISEASE:
Genetic variations in CR2 are associated with susceptibility to systemic lupus erythematosus type 9 (SLEB9) [MIM:610927]. Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with a complex genetic basis. SLE is an inflammatory, and often febrile multisystemic disorder of connective tissue characterized principally by involvement of the skin, joints, kidneys, and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
Defects in CR2 are the cause of immunodeficiency, common variable, type 7 (CVID7) [MIM:614699]. A primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B cells is usually in the normal range, but can be low.

Similarity:
Belongs to the receptors of complement activation (RCA) family.
Contains 15 Sushi (CCP/SCR) domains.

SWISS:
P20023

Gene ID:
1380

Database links:

Entrez Gene: 1380 Human

Entrez Gene: 12902 Mouse

Omim: 120650 Human

SwissProt: P20023 Human

SwissProt: P19070 Mouse

Unigene: 445757 Human

Unigene: 235387 Mouse



產(chǎn)品圖片
Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-CD21 (bs-3792R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 111 kD Observed band size: 111 kD
Blank control(blue): U-87MG Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (bs-3792R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-CD21/AF647 Conjugated antibody (bs-3792R/AF647), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.
Blank control: Raji cells(blue). Primary Antibody:Rabbit Anti- CD21 antibody(bs-3792R), Dilution: 1μg in 100 1μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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