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Rabbit Anti-FZD3  antibody (bs-13239R)
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產(chǎn)品編號 bs-13239R
英文名稱 Rabbit Anti-FZD3  antibody
中文名稱 卷曲蛋白3抗體
別    名 Frizzled 3; Frizzled 3 seven transmembrane spanning receptor; Frizzled family receptor 3; Frizzled homolog 3 (Drosophila); Frizzled homolog 3; Frizzled-3; Frizzled3; Fz 3; Fz-3; Fz3; FZD 3; Fzd3; FZD3_HUMAN; hFz 3; hFz3.  
Specific References  (1)     |     bs-13239R has been referenced in 1 publications.
[IF=2.64] Su, Lin, et al. "Deregulation of the planar cell polarity genes CELSR3 and FZD3 in Hirschsprung disease." Experimental and Molecular Pathology(2016).  IHC-P ;  Human.  
研究領(lǐng)域 細胞生物  信號轉(zhuǎn)導  干細胞  G蛋白偶聯(lián)受體  G蛋白信號  
抗體來源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 74kDa
細胞定位 細胞膜 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human FZD3/Frizzled 3: 121-220/666 <Extracellular>
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 This gene is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development. Alternative splicing results in multiple transcript variants. This gene is a susceptibility locus for schizophrenia. [provided by RefSeq, Dec 2010]

Function:
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues.

Subunit:
Interacts with VANGL2.

Subcellular Location:
Membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue Specificity:
Widely expressed. Relatively high expression in the CNS, including regions of the limbic system, in kidney, pancreas, skeletal muscle, uterus and testis.

Post-translational modifications:
Ubiquitinated by ZNRF3, leading to its degradation by the proteasome.

Similarity:
Belongs to the G-protein coupled receptor Fz/Smo family.
Contains 1 FZ (frizzled) domain.

SWISS:
Q9NPG1

Gene ID:
7976

Database links:

Entrez Gene: 7976 Human

Entrez Gene: 14365 Mouse

Entrez Gene: 100153078 Pig

Entrez Gene: 100339544 Rabbit

Entrez Gene: 266715 Rat

Entrez Gene: 395572 Chicken

Entrez Gene: 445419 Cow

Entrez Gene: 486093 Dog

Entrez Gene: 100054732 Horse

Omim: 606143 Human

SwissProt: Q9NPG1 Human

SwissProt: Q61086 Mouse

Unigene: 40735 Human

Unigene: 214687 Mouse

Unigene: 404299 Mouse

Unigene: 178695 Rat



產(chǎn)品圖片
Protein: pancreas(mouse) lysates at 30ug; Primary: Anti-FZD3 (bs-13239R) at 1:300; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kD
Protein: brain(Rat) lysates at 30ug; Primary: Anti-FZD3 (bs-13239R) at 1:300; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 74kD Observed band size : 74kD, 25kD
Sample: Lane 1: Human SW480 cell Lysates Lane 2: Human U-2 OS cell Lysates Lane 3: Human A431 cell Lysates Lane 4: Human SH-SY5Y cell Lysates Primary: Anti-FZD3 (bs-13239R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 74kDa Observed band size: 70kDa
Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FZD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-FAD3 Polyclonal Antibody, Unconjugated(bs-13239R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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