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Rabbit Anti-ROR alpha  antibody (bs-17164R)
~~~促銷代碼KT202409~~~
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產(chǎn)品編號(hào) bs-17164R
英文名稱 Rabbit Anti-ROR alpha  antibody
中文名稱 維甲酸相關(guān)孤兒受體α抗體
別    名 NR1F1; Nuclear receptor ROR-alpha; Nuclear receptor RZR-alpha; Nuclear receptor subfamily 1 group F member 1; RAR-related orphan receptor A; Retinoid-related orphan receptor-alpha; Rora; RORA_HUMAN; RZR-ALPHA; RZRA.  
Specific References  (2)     |     bs-17164R has been referenced in 2 publications.
[IF=5.542] Han L et al. The RNA‐binding protein SORBS2 suppresses hepatocellular carcinoma tumorigenesis and metastasis by stabilizing RORA mRNA. Liver Int. 2019 Jul 31.  IHC ;  Human.  
[IF=2.923] Hongwei Duan. et al. Melatonin regulates dihydrotestosterone formation via its membrane receptor in the epididymal epithelial cells of sheep. THERIOGENOLOGY. 2022 Dec;:  WB ;  Sheep.  
研究領(lǐng)域 腫瘤  細(xì)胞生物  神經(jīng)生物學(xué)  信號(hào)轉(zhuǎn)導(dǎo)  生長(zhǎng)因子和激素  細(xì)胞周期蛋白  細(xì)胞分化  
抗體來(lái)源 Rabbit
克隆類型 Polyclonal
交叉反應(yīng) Human,Mouse,Rat
產(chǎn)品應(yīng)用 WB=1:500-1000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 59kDa
細(xì)胞定位 細(xì)胞核 
性    狀 Liquid
濃    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human ROR alpha: 151-250/523 
亞    型 IgG
純化方法 affinity purified by Protein A
緩 沖 液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4
保存條件 Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項(xiàng) This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The encoded protein has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Also, it has been shown to aid in the transcriptional regulation of some genes involved in circadian rhythm. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2014]

Function:
Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives.

Subunit:
Monomer.

Subcellular Location:
Nucleus.

Tissue Specificity:
Widely expressed in a number of tissues. Expressed in both regulatory T-cells (Treg) and effector T-cells (Teff).

Similarity:
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.

SWISS:
P35398

Gene ID:
6095

Database links:

Entrez Gene: 6095 Human

Omim: 600825 Human

SwissProt: P35398 Human

Unigene: 560343 Human

Unigene: 655155 Human



產(chǎn)品圖片
Sample: Jurkat (Human) Cell Lysate at 40 ug Primary: Anti-ROR alpha(bs-17164R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59 kD
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROR alpha (bs-17164R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Sample: A431(human) Cell Lysate at 40 ug Primary: Anti-ROR alpha (bs-17164R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Sample: Lane 1: Mouse Heart tissue lysates Lane 2: Rat Heart tissue lysates Lane 3: Human HeLa cell lysates Primary: Anti-ROR alpha (bs-17164R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ROR alpha ) Polyclonal Antibody, Unconjugated (bs-17164R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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