| 產品編號 | bs-41452P |
| 英文名稱 | Recombinant SARS-Cov-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F), His |
| 中文名稱 | 重組SARS冠狀病毒核衣殼突變蛋白(D3L,P13T,D103Y,D128Y,H145Y,R203K,G204R,T205I,S235F), His tag, (南非) |
| 別 名 | SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein, 2019-nCOV Nucleocapsid protein (D3L,P13T,D103Y,D128Y,H145Y,R203K,G204R,T205I,S235F),Recombinant (His Tag). ;Recombinant SARS-Cov-2 N protein (D3L, P13T, D103Y, D128Y, H145Y, R203K, G204R, T205I, S235F), His 突變型新冠蛋白; 新冠病毒N蛋白突變體（D3L,P13T,D103Y,D128Y,H145Y,R203K,G204R,T205I,S235F）; 重組新冠病毒核衣殼突變蛋白(D3L,P13T,D103Y,D128Y,H145Y,R203K,G204R,T205I,S235F); 新型冠狀病毒核衣殼突變體蛋白. |
| 理論分子量 | 46kDa |
| 檢測分子量 | 50 kDa |
| 性 狀 | Lyophilized or Liquid |
| 濃 度 | ＞0.5 mg/ml |
| 物 種 | SARS-Cov-2 |
| 序 列 | 1-419/419 |
| 純 度 | >90% as determined by SDS-PAGE |
| 純化方法 | AC |
| 內毒素 | Not analyzed |
| 表達系統(tǒng) | E.coli |
| 活性 | Not tested |
| 標簽 | His |
| 緩 沖 液 | 20 mM Tris-HCl (pH=8.0) with 500 mM NaCl |
| 保存條件 | Stored at -70℃ or -20℃. Avoid repeated freeze/thaw cycles. |
| 注意事項 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 產品介紹 |
Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool. SWISS: P0DTC9 Gene ID: 43740575 |
| 產品圖片 |
The purity of the protein is greater than 90% as determined by reducing SDS-PAGE.
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| 1、抗體溶解方法 | |
| 2、抗體修復方式 | |
| 3、常用試劑的配制 | |
| 4、免疫組化操作步驟 | |
| 5、免疫組化問題解答 | |
| 6、Western Blotting 操作步驟 | |
| 7、Western Blotting 問題解答 | |
| 8、關于肽鏈的設計 | |
| 9、多肽的溶解與保存 | |
| 10、酶標抗體效價測定程序 | |
