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Mouse Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid  antibody (bsm-41414M)
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產(chǎn)品編號 bsm-41414M
英文名稱 Mouse Anti-SARS-CoV-2 (2019-nCoV) Nucleocapsid  antibody
中文名稱 SARS冠狀病毒2核衣殼蛋白單克隆抗體
別    名 SARS-CoV-2 Nucleocapsid Protein; SARS-CoV-2 NP; nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2]; novel coronavirus N Protein; novel coronavirus Nucleocapsid Protein; 2019-nCoV Nucleoprotein; 2019-nCoV N; 2019nCoV N; 2019-nCoV N Protein; 2019 ncov N Protein; 2019-nCoV nucleocapsid protein.  
Specific References  (5)     |     bsm-41414M has been referenced in 5 publications.
[IF=7.344] Lassaunière, Ria. et al. Preclinical evaluation of a candidate naked plasmid DNA vaccine against SARS-CoV-2. Npj Vaccines. 2021 Dec;6(1):1-13  ELISA ;  Mouse.  
[IF=5.897] Larry L. Luchsinger. et al. Serological Assays Estimate Highly Variable SARS-CoV-2 Neutralizing Antibody Activity in Recovered COVID19 Patients. J Clin Microbiol. 2020 Nov;58(12):e02005-20  ELISA ;  Human.  
[IF=3.752] Anders Frische. et al. Optimization and evaluation of a live virus SARS-CoV-2 neutralization assay. PLOS ONE. 2022 Jul;17(7):e0272298  ELISA ;  Monkey.  
[IF=3.09] Nesbitt, Daniel J.. et al. Low Seroprevalence of SARS-CoV-2 in Rhode Island blood donors during may 2020 as determined using multiple serological assay formats. Bmc Infect Dis. 2021 Dec;21(1):1-9  ELISA ;  Human.  
[IF=2.74] Daniel K. Jin. et al. Seroprevalence of anti-SARS-CoV-2 antibodies in a cohort of New York City metro blood donors using multiple SARS-CoV-2 serological assays: Implications for controlling the epidemic and “Reopening”. Plos One. 2021 Apr;16(4):e0250319  ELISA ;  
抗體來源 Mouse
克隆類型 Monoclonal
克 隆 號 7E1B
交叉反應(yīng) SARS-CoV-2 (predicted: SARS-CoV-2)
產(chǎn)品應(yīng)用 WB=1:500-2000,ELISA=1:5000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量 46kDa
性    狀 Liquid
濃    度 ≥1 mg/ml
免 疫 原 Recombinant SARS-CoV-2 Nucleocapsid Protei: full length  
亞    型 IgG2b
純化方法 affinity purified by Protein G
緩 沖 液 0.01M PBS(pH7.4) with 0.1% Proclin300
保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
注意事項 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
產(chǎn)品介紹 Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

SWISS:
P0DTC9

Gene ID:
43740575

產(chǎn)品圖片
Sample: Lane 1: SARS-CoV-2 N protein(Cat# bs-41408P) at 50ng Lane 2: SARS-CoV-2 N protein(Cat# bs-41408P) at 25ng Lane 3: SARS-CoV-2 N protein(Cat# bs-41408P) at 5ng Lane 4: SARS-CoV-2 N protein(Cat# bs-41408P) at 2ng Lane 5: SARS-CoV-2 N protein(Cat# bs-41408P) at 1ng Lane 6:SARS-CoV-2 N protein(Cat# bs-41408P) at 0.5ng Primary: Anti- SARS-CoV-2 N protein (bs-41414M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 46 kD Observed band size: 46 kD
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